Journal: eLife
Article Title: Enkephalin-mediated modulation of basal somatic sensitivity by regulatory T cells in mice
doi: 10.7554/eLife.91359
Figure Lengend Snippet: Representative staining of TdTomato and mCherry in naive (CD62L high CD44 low ), central memory (CD62L high CD44 high ), and effector memory (CD62L low CD44 high ) Tconv (top) and Treg (bottom) in the lymph nodes 1-week post-Tamoxifen (TMX) administration.
Article Snippet: For flow cytometry staining, after Fc receptor saturation (anti-mouse CD16/32, clone 93, 1:50, Biolegend) and dead cells detection (Fixable Viability Dye, 1:1000, Thermo Fisher), cells were surface labeled for 30 min on ice with the following antibodies: CD8a-BV421 (Clone 53-6.7, 1:200, BD Biosciences #563898), CD44-BV605 (Clone IM7, 1:200, BD Biosciences #563058), CD3e-BV711 (Clone 145-2C11, 1:50, Biolegend #100349), CD4-PE-CF594 (Clone RM4-5, 1:800, BD Biosciences #562285), and CD62L-PE-Cy7 (Clone MEL-14, 1:300, BD Biosciences #560516).
Techniques: Staining